Recent policies have mandated that a portion of biofuels be produced from lignocellulosic biomass, which includes agricultural residues e. Our objective was to quantify nutrient removal, carbohydrate composition, theoretical ethanol yield tey for various stover fractions. Inorganic salts, nacl, kcl, cacl2, mgcl2, fecl2, feso4, fecl3, and fe2so43, were studied as catalysts for the degradation of hemicellulose in corn stover. Jun 17, 2009 corn stover, the aboveground, nongrain portion of the crop, is a large, currently available source of biomass that potentially could be collected as a biofuels feedstock. Thermogravimetric characterization of corn stover as gasi. Corn stover also referred to as maize stover is an agricultural residue similar to straw that is considered to be a substantial potential biorefining resource in the usa an availability of 200 million tonnes has been estimated, equivalent to more than half the total amount of agricultural residues produced. Corn stover was ground until the entire material passed through a 2mm sieve, and then it was sieved to obtain 40 oto 60mesh fractions and stored at. As the corn plant matures, the proportion of lignin increases to keep the plant upright in the field prior to harvest, and the more digestible cellulose and hemicellulose fractions decrease. Corn stover is made up of the stalk, leaves, husks and tassels left in the field after harvesting the grain with a combine. C in an oven for 12 h, the corn cob was cut into small pieces cm, milled in a knives mill to pass through a 0. The types of monomers involved in the polymerization of hemicellulose are xylose, galactose, mannose, rhamnose, and arabinose.
Experimental materials and methods corn hulls from zea mays l. Airdried corn stover was collected from suihua, heilongjiang province, china. Hydrothermal treatments of corn cob and hemicelluloses extraction. Pdf hemicelluloses were extracted from corn stover with potassium hydroxide and were further delignified with hydrogen peroxide. Once the corn stover enters the plant it must be handled and pretreated. However, like other sources of lignocellulosic biomass, even from a single species, it is heterogeneous in nature due to the different tissues and cell types, and this has implications for saccharification efficiency. Complicating the molecular picture of lignin further is the fact that. Determination of hemicellulose, cellulose and lignin in moso. Analysis of corn stover, cellulose content of corn stover, lignin. To be competitive with existing subsidized fuels, ethanol facilities need to be efficient and economical.
A hemicellulose also known as polyose is one of a number of heteropolymer matrix polysaccharides, such as arabinoxylans, present along with cellulose in almost all terrestrial plant cell walls. The elemental compositions obtained by other researchers were similar, but the composition of cellulose, hemicellulose and lignin varied from 28% to 51%, 19. Elemental analysis of corn stalk and hemicellulose were determined. Corn stover is a very common agricultural product in areas of large amounts of corn production. Fractionation of lignocellulosic biomass is an important process in producing biofuels.
Such stover makes up about half of the yield of a corn crop 1 and is similar to straw from other cereal grasses. Assessing corn stover composition and sources of variability via nirs. Corn residues corn cob were kindly supplied by a local farmer portugal. Yu, in handbook of waste management and coproduct recovery in food. Corn stover pretreatment by inorganic salts and its effects. Hemicellulose hydrolyzate containing 34 gl xylose, 8 gl glucose, 8 gl acetic acid, 0. Fecl3 significantly increased the hemicellulose degradation in aqueous solutions heated between 140 and 200c with high xylose recovery and low cellulose removal, amounting to. Corn stover consists mostly of cellulose, hemicelluloses, and lignin chen et al. The composition of corn stover is provided in table 1, and it is compared to the composition of corn grain, corn silage, and wheat straw. Cellulose is homogenous and composed of glucose monomers linked by glycosidic bonds. Pretreatment and fractionation of corn stover by soaking in ethanol and aqueous ammonia. More specifically, the stover must go through a hydrolysis, fermentation, and distillation process in order to produce. Although the composition of corn fiber varies by source, cellulose and hemicellulose typically account for about 50% of the dry weight, with adherent starch comprising another 10 20%.
The composition and structure of the hemicellulose in the softwood differ in a characteristic way from those in the heartwood. The chemical composition of whole corn stover and its morphological fractions were assayed as follows. Stover is the corn plant residues typically left in the field after corn grain harvestthe cobs, husks, leaves, and stalks. Effect of extractives on digestibility of cellulose in. Variation in the chemical composition of wheat straw.
Biomass ethanol technology is still developing and important questions need to be answered about corn stover removal, but prospects are excellent for you to someday be able to harvest and sell a substantial portion of your stover for fuel productionwith\ out hurting your soil or main. This stover can be used to make advanced biofuels or be used as a low quality, emergency livestock feed. Characterization of chemical composition, milling properties and carbon dioxide diffusivity resulting from early harvest corn and corn stover by haibo huang dissertation submitted in partial fulfillment of the requirements for the degree of doctor of philosophy in agricultural and biological engineering in the graduate college of the. These differences can impact optimal pretreatment conditions, enzymatic digestibility and maximum achievable sugar yields in the process of converting lignocellulosics to ethanol. While cellulose is crystalline, strong, and resistant to hydrolysis, hemicelluloses have random, amorphous structure with little strength. Comparative study of corn stover pretreated by dilute acid. Schell using a series of thermochemical pretreatment and biochemical steps, lignocellulosic biomass is converted to sugars that subsequently can be made into fuels and chemicals. Given these disadvantages, depithed corn stover was used in this study for hemicellulose extraction and sodaaq anthraquinone pulping, noting that the pith from the corn stover can be used as animal feed. Hemicellulose content was higher in corn stover and bmrsorghum, than in nonbmr sorghum. Assessing corn stover composition and sources of variability.
Carbohydrate and nutrient composition of corn stover from. In this multiyear study we found that of the stover material remaining after harvest roughly 55% is found in the stalk and leaf material below the ear, 25% in the stalk and leaf material. Corn fiber as a raw material for hemicellulose and ethanol. The fractions having particle sizes between 10 mesh and 35 mesh 0. Corn cobs hemicelluloses isolation method comparison and. The cellulose and hemicellulose components in corn stover are long chain polymers of. N2 corn fiber cf is a potential raw material for the production of various products because it is widely available in cornproducing countries. Corn stover, as any form of plant biomass, is composed of cellulose, hemicellulose, lignin, ash, and extractives 4. The composition of corn stover and wheat straw is somewhat similar and are similar in price values at the reported dm table 1, but at similar dm e. The ground and screened corn stover was air dried and stored at about 25 c, which was the. The hemicellulose was isolated using a modified method as previously described lv et al. The results showed that the yield of corn cobs hemicellulose with characteristic hplc chromatograms from all procedures of isolation had a.
Impact of corn stover composition on hemicellulose. Extraction and characterization of native heteroxylans from. Pdf hemicelluloses were extracted from corn stover with potassium hydroxide and were. T1 corn fiber as a raw material for hemicellulose and ethanol production. The direct conversion of both cellulose and hemicellulose in raw corn stover to furfural. Hydrothermal treatments of corn cob and hemicelluloses. Under such conditions, 91% of hemicellulose was removed, and the recovery of monomeric and oligomeric xylose in liquid fraction achieved 89%, meanwhile, only 9% of cellulose was removed. Cellulose and hemicellulose decomposition by forest soil. Lignocellulosic biomass, such as corn stover, contains 6070% dry weight carbohydrates. Contents lists available at sciencedirect biomass and bioenergy. Production of ethanol from corn stover hemicellulose.
Pretreatment and fractionation of corn stover by soaking. Biomass conversion process economics are directly affected by the overall biochemical conversion yield, which is assumed to be proportional to the carbohydrate content of the feedstock materials used in the process. Hopkins, 1900, university of illinois agricultural experiment station edition. Thus, corn fiber typically consists of about 70% recoverable sugars. Corn stover corn stover was obtained from the national renewable energy laboratory nrel in golden, co, usa. Corn cobs hemicelluloses isolation method comparison and its. Physicochemical properties, including chemical structure, composition, molecular weight and thermal stability, of the extracted hemicelluloses. Enzymatic hydrolysis, supramolecular structure, and substrate accessibility, author zhu, z and sathitsuksanoh, n and vinzant, t and schell, d j and mcmillian, j d and zhang, y h. Optimizing harvest of corn stover fractions based on. Chemical composition, structure, and response to enzyme pretreatment february 2006 applied biochemistry and biotechnology 12921. Pretreatment and fractionation of corn stover by soaking in.
Isolation and characterization of hemicelluloses of corn hulls. The composition of the corn stover is shown in table 1. Dec, 2011 composition and digestibility of corn fodder and corn stover by cyril g. Mosier a,1, rick hendrickson a,1, thaddeus ezeji b,2, hans blaschek b,2, bruce dien c,3, michael cotta c,3, bruce dale d,4, michael r. Corn stover composition changes considerably throughout the growing season and also varies between the various fractions of the plant. The feasibility of using near infrared nir spectroscopy for rapid. Impact of corn stover composition on hemicellulose conversion during dilute acid pretreatment and enzymatic cellulose digestibility of the pretreated solids. The pretreatment of forage sorghum biomass at 160c, 10 min and 1, wt% acid concentration, led to maximum hemicellulose and cellulose hydrolysis. Compared to fossil fuel, it is abundant, renewable and environmental friendly, while compared to 1 st generation feedstock, like corn, it does not compete with world food supply 1, 2. Corn fibre as a raw material for hemicellulose and ethanol. Yield and moisture content of corn stover components in. Improving the feeding value of corn stover is a challenge because, as a byproduct of corn grain production, the corn plant has a high lignin content. Corn stover consists of the leaves, stalks, and cobs of maize corn zea mays ssp. Optimum pretreatment conditions were found where the corn stover was pretreated with 0.
The chemical composition of corn stover is quite variable from lot to lot, and there are also indications that different lots of stover exhibit. Corn stover contains principally cellulose and hemicellulose, which are the main sources of fermentable sugars for ethanol production. Acid hydrolysis of corn stover hemicellulose by lowliquid. The initial composition of corn stover was assayed by the national renewable energy laboratory nrel. Some things that must be considered when feeding corn stover are. Estimating a value for corn stover iowa state university. The grain and forage components of corn are low in protein, but they especially contribute energy to the diet and in the case for the forage component, fiber for ruminal health. Hemicellulose refers to a constituent of the plant cell wall that consists of a simple structure than cellulose. For instance, xylan content is usually greatest in the cob whilst the husks tend to have more arabinose than in present in the other fractions. Assessment of the nutritive value of whole corn stover and. Agricultural residues corn stover, sugarcane bagasse, spent sugar beet pulp and sweet sorghum, etc. Pdf separation, purification and characterization of corn stover. Primarily the corn kernel is used for ethanol production.
Variations of sugar content in the hydrolysate under different holding time were detected. Tables 1 and 2 summarize the composition of corn stover. Corn grain includes the starchy endosperm, germ, and corn hull. The biomass was delignified by two different techniques. Analysis of corn stover structural properties for mechanical processing applications,the journal of undergraduate research. Evaluation and prediction of corn stover biomass and. A mineral acidfree approach hilda gomez bernal,a luca bernazzania and anna maria raspolli gallettia,b furfural was obtained from corn stover hemicelluloses by a microwaveassisted, green and heterogeneously catalyzed twostep cascade process as follows.
Dimethylsulfoxidesolubilized polysaccharides from delignified corn stover and aspen were characterized. Analysis of corn stover, cellulose content of corn stover. For efficient hydrolysis of the two carbohydrate fractions by enzymes normally a. Such stover makes up about half of the yield of a corn crop and is similar to straw from other cereal grasses.
Corn stover is the stalks, leaves, husks, tassels, and cobs of the corn plant remaining after removal of the corn kernels. Ladisch a, a laboratory of renewable resources engineering, potter engineering center, 500 central. Impact of corn stover composition on hemicellulose conversion during dilute acid pretreatment and enzymatic cellulose digestibility of the pretreated solids author. Impact of corn stover composition on hemicellulose conversion. Ddgs, wet cake, and thin stillage youngmi kim a,1, nathan s. There are a number of factors you should consider before you set a price for corn stover. Corn residue has a number of uses and thus its value as well as its impact on other systems may need to be estimated when evaluating postharvest options. Thermogravimetric characterization of corn stover as. Analysis of corn stover structural properties for mechanical processing applications taylor suess. Unlocking the potential of corn stover progressive dairy. Variation in corn stover composition and energy content.
As established in the previous section, corn has the least expensive total cost for ethanol production. Feeding corn stover to ruminants ohio dairy industry. Separate processing of hemicellulose and cellulose to optimize ethanol production from corn stover alexandre chapeaux, nancy dowe, daniel j. Corn stover contains principally cellulose and hemicellulose, which are. Separate processing of hemicellulose and cellulose to. Characteristics of corn stover components pyrolysis at. Typically, these fuels have been produced from corn grain ethanol and soybeans biodiesel. The major components of lignocellulosic biomass are cellulose, hemicellulose and lignin. Forestry residues fallen branches, leaves, twigs, saw dust, etc. Production of cellulase from corn cobs by under submerged. Federal policies require that biofuels, such as ethanol and biodiesel, be blended into petroleumbased transportation fuels. Composition of corn and distillers dried grains with solubles from dry grind ethanol processing r. Riegels handbook of industrial chemistry and biotechnol ogy, vol 2. In fact, the corn stover component concentration varies depending on.
Apr 29, 2016 again, there is an important difference between endo and exocleaving enzymes, but the complexity of hemicellulose composition typically requires the action of a wide set of enzymes such as the endoxylanases, endomannanases, xylosidases, glucosidases, arabinosidases, galactosidases, mannosidases and glucuronidases. Some corn producers have stover to sell but do not have a baler. The yield and composition of hemicellulose polysaccharides were measured to investigate the effect of extraction temperature and. Harvest index is defined as the pounds of grain divided by the total pounds of above ground biomass stover plus grain. Considerable differences also exist in the hemicellulose content and composition between the.
This accounts for transporting bales from the farm to the biorefinery as well as a return trip of the transporting vehicle backhaul. Due to the fibrous composition of corn stover, the bending strength of the stalk in general is. Composition of corn and distillers dried grains with solubles. At higher solids loadings 150200 g dry stover per liter, sulfuric acid catalyzed hydrolysis results in more. Full text of composition and digestibility of cornfodder. These results, along with scanning electron microscopy images showing dramatic structural differences between the da. Physical pretreatments do not alter the chemical composition, but mainly. This article looks at how to estimate the nutrient value of the residue and potential impacts to the soil from removing the residue, based on nebraska research. Hemicellulose hemicellulose long branched sugar chains polymer, polysaccharide amorphous composition varies largely from species to species c6 andor c5 sugars lignin binder 30% hemicellulose various sugars 25% cellulose fiber 45%. Sugar content in corn stover after preheating and waterwashing preheating time 25 minutes, final temperature 180oc sugar content retained in the solid after washing sugars in wash water estimated sugar loss by decomposition glucan 95.
Although the composition of agricultural crop wastes is different, the typical percentage of the dominant chemical composition in corn stover, rice straw, and wheat straw is. Nov 25, 2015 the contents of hemicellulose, cellulose and lignin are important for moso bamboo processing in biomass energy industry. Aug 20, 2014 wheat straw is an attractive substrate for second generation ethanol production because it will complement and augment wheat production rather than competing with food production. Corn stover, as any form of plant biomass, is composed of cellulose, hemicellulose, lignin, ash, and extractives. The composition ww of corn stalk used was as follows. For explanations of technical terms and information concerning the uses of food constituents the reader is referred to the appendix to bulletin no. Process development for the fractionation and isolation of corn fiber hemicellulose a thesis presented to the graduate school of clemson university in partial fulfillment of the requirements for the degree master of science biosystems engineering by justin matthew montanti august 2010 accepted by.
They may prefer to sell unharvested stover and let the buyer do the harvesting. The fermentation rate increased with aeration but the ph also increased due to consumption of acetic acid by pichia stipitis. Part of theagricultural science commons,agriculture commons, and theagronomy and crop sciences commons. Corn fibre as a raw material for hemicellulose and ethanol production article in process biochemistry 427. The corn cob contains more cellulose and hemicellulose than stalks and leaves. Thus, it was established that the corn stover composition has huge variations. The compositions of ashes and extractives in the intact and pretreated biomass samples were measured according to. Maize cobs are rich in fibre and poor in protein, and are thus a lowquality roughage. The objective was to document variations in corn stover composition just before and after a typical corn grain harvest period in eastern canada.
This resource is so large partially because of the amount of corn grown in. Despite this low value, their wide availability, large quantities, easy and cheap procurement, and also their large cellulose and hemicellulose reserves enhance their utilization as energy sources in ruminant feed, particularly in areas or during times where better ingredients are not available adebowale. Corn stover also referred to as maize stover is an agricultural residue similar to straw that. Cellulose and hemicellulose are the most desirable portions of biomass for bioethanol conversion. Hemicellulose the amount of hemicellulose of the dry weight of wood is usually between 20 and 30%. As such, lignin is the second most abundant natural polymer on earth fox 2006. These results formed the basis for optimizing the hydrolysis of hemicellulose from corn stover using maleic acid. Separation, purification and characterization of corn. While this book chapter separates ethanol yields from starch and ligno. Mar 21, 2014 lignocellulosic biomass, like bagasse, wheat straw, and corn stover, is the 2 nd generation feedstock for biofuel production.
According to the us department of energy eere biomass program 2012, cellulosic biomass can be obtained from a variety of sources, such as. Hemicellulose content of corn stover the total hemicellulose content, and its constituent sugars, varies greatly according to the stover fraction. Lignin, along with cellulose and hemicellulose, is a major component of the compositelike system comprised entirely of natural polymers responsible for the strength and rigidity of plant stems. Tumbleson c a department of animal sciences, university of missouri, 115 asrc, 920 east campus drive, columbia, mo 65211, usa. Hemicellulose liquid hydrolyzate from dilute acid pretreated corn stover was fermented to ethanol using pichia stipitis cbs 6054. Identifying inhibitory compounds in lignocellulosic biomass. The results showed that the yield of corn cobs hemicellulose with characteristic hplc chromatograms from all procedures of isolation had a retention time of 1.
The research clearly demonstrates that 1 the chemical composition of the different anatomical fractions of corn stover differ from each other at the same time in plant development, and 2 the chemical composition of individual anatomical fractions changes even during the end period of the corn plants life cycle. The contents of furfural and 5hydroxymethyl2furaldehyde generated during the extraction were also determined. Composition and digestibility of cornfodder and corn stover. Evaluation and prediction of corn stover biomass and composition. The main aboveground components of corn grain, stalk, leaf, husk. Biomimetic catalysis for hemicellulose hydrolysis in corn. In 2009, 2010, and 2011, wholeplant samples were collected from one field study in south carolina sc and two in alabama al. Conversion of cellulose, hemicellulose and lignin into. Variation in corn stover composition and energy content with. Evaluation and prediction of corn stover biomass and composition from commercially available corn hybrids ty jason barten iowa state university follow this and additional works at.
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